Natural Products Biotechnology Group

Group Leader: Dr. Mattijs Julsing

Research Focus

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Members

Group Leader
 
Dr. Mattijs Julsing 
PostDocs and PhD Students Undergraduate Students
Kerstin LangeMartin Becker
Christian WillrodtMarina Breisch
  
  
  
Publications
Peer Reviewed Articles
2016
  • Kadisch M., Julsing M. K., Schrewe M., Jehmlich N., Scheer B., von Bergen M., Schmid A. and Bühler B. (2016)
    Cell viability rather than enzyme kinetics determines whole-cell ω-oxyfunctionalization performance.
    Biotechnology & Bioengineering, Accepted
  • Willrodt C., Hoschek A., Bühler B., Schmid A. and Julsing M. K. (2016)
    Decoupling production from growth by magnesium sulfate limitation boosts de novo limonene production.
    Biotechnology and Bioengineering, 113(6):1305-1314, DOI-Link
  • Ladkau N., Aßmann M., Schrewe M., Julsing M. K., Schmid A. and Bühler B. (2016)
    Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli.
    Metabolic Engineering, 36:1-9, DOI-Link
2015
  • Lange K., Schmid A. and Julsing M. K. (2015)
    Δ-9-Tetrahydrocannabinolic acid synthase production in Pichia pastoris enables chemical synthesis of cannabinoids .
    Journal of Biotechnology, 211:68–76, DOI-Link
  • Lange K., Poetsch A., Schmid A. and Julsing M. K. (2015)
    Enrichment and identification of Δ-9-tetrahydrocannabinolic acid synthase from Pichia pastoris culture supernatants
    .
    Data in Brief, 4:641-649, DOI-Link, Pubmed
  • Willrodt C., Hoschek A., Bühler B., Schmid A. and Julsing M. K. (2015)
    Coupling limonene formation and oxyfunctionalization by mixed-culture resting cell fermentation.
    Biotechnology & Bioengineering, 112(9):1738-1750, DOI-Link
  • Looße C., Galozzi S., Debor L., Julsing M. K., Bühler B., Schmid A., Barkovits K., Müller T. and Marcus K. (2015)
    Direct infusion-SIM as fast and robust method for absolute protein quantification in complex samples.
    EuPA Open Proteomics, 7:20-26, DOI-Link
  • Willrodt C., Karande R., Schmid A. and Julsing M. K. (2015)
    Guiding efficient microbial synthesis of non-natural chemicals by physicochemical properties of reactants.
    Current Opinion in Biotechnology, 35:52-62, DOI-Link
2014
  • Willrodt C., David C., Cornelissen S., Bühler B., Julsing M. K. and Schmid A. (2014)
    Engineering the productivity of recombinant Escherichia coli for limonene formation from glycerol in minimal media.
    Biotechnology Journal, 9(8):1000–1012, DOI-Link
  • Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
    Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
    Biotechnology and Bioengineering, 111(9):1820-1830, DOI-Link
2013
  • Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
    Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
    Chemical Society Reviews, 42(15):6346 - 6377, DOI-Link
    * These authors contributed equally to this work.
  • Cornelissen S., Julsing M. K., Volmer J., Riechert O., Schmid A. and Bühler B. (2013)
    Whole-cell-based CYP153A6-catalyzed (S)-limonene hydroxylation efficiency depends on host background and profits from monoterpene uptake via AlkL.
    Biotechnology and Bioengineering, 110(5):1282-1292, DOI-Link
2012
  • Cornelissen S., Julsing M. K., Schmid A. and Bühler B. (2012)
    Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis.
    Journal of Industrial Microbiology and Biotechnology, 39(2):275-287, DOI-Link
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
    Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
    Biotechnology and Bioengineering, 109(5):1109-1119, DOI-Link
  • Kuhn D., Julsing M. K., Heinzle E. and Bühler B. (2012)
    Systematic optimization of a biocatalytic two-liquid phase oxyfunctionalization process guided by ecological andeconomic assessment.
    Green Chemistry, 14(3):645 - 653, DOI-Link
  • Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
    Applied and Environmental Microbiology, 78(16):5724-5733, DOI-Link
    * These authors contributed equally to this work.
2008
  • Julsing M. K., Fichera M. A., Malz F., Ebbelaar M., Bos R., Woerdenbag H. J., Quax W. J. and Kayser O. (2008)
    Bioconversion of mono- and sesquiterpenoids by recombinant human cytochrome P450 monooxygenases.
    Pharmaceutical Biology, 46(10):710-718, DOI-Link
  • Julsing M. K., Cornelissen S., Bühler B. and Schmid A. (2008)
    Heme-iron oxygenases: powerful industrial biocatalysts?
    Current Opinion in Chemical Biology, 12(2):177-186, DOI-Link, PubMed
  • Julsing M. K., Vasilev N.P., Schneidman-Duhovny D., Muntendam R., Woerdenbag H. J., Quax W. J., Wolfson H. J., Ionkova I. and Kayser O. (2008)
    Metabolic stereoselectivity of cytochrome P450 3A4 towards deoxypodophyllotoxin: In silico predictions and experimental validation.
    European Journal of Medicinal Chemistry, 43(6):1171-1179, DOI-Link, PubMed
2007
  • Julsing M. K., Rijpkema M., Woerdenbag H. J., Quax W. J. and Kayser O. (2007)
    Functional analysis of genes involved in the biosynthesis of isoprene in Bacillus subtilis.
    Applied Microbiology and Biotechnology, 75(6):1377-84, DOI-Link, PubMed
2006
  • Vasilev N.P., Julsing M. K., Koulman A., Clarkson C., Woerdenbag H. J., Ionkova I., Bos R., Jaroszewski J. W., Kayser O. and Quax W. J. (2006)
    Bioconversion of deoxypodophyllotoxin into epipodophyllotoxin in E. coli using human cytochrome P450 3A4.
    Journal of Biotechnology, 126(3):383-93, DOI-Link, PubMed
  • Julsing M. K., Koulman A., Woerdenbag H. J., Quax W. J. and Kayser O. (2006)
    Combinatorial biosynthesis of medicinal plant secondary metabolites .
    Biomolecular Engineering, 23(6):265-79, DOI-Link, PubMed
  • Dröge M. J., Boersma Y.L., Braun P. G., Buining R. J., Julsing M. K., Selles K. G., van Dijl J. M. and Quax W. J. (2006)
    Phage display of an intracellular carboxylesterase of Bacillus subtilis: comparison of Sec and Tat pathway export capabilities.
    Applied and Environmental Microbiology, 72(7):4589-95, DOI-Link, PubMed
Books & Bookchapters
2007
  • Kayser O. and Julsing M. K. (2007)
    New drug leads in the pipeline of antiparasitic drug research.
    in Melhorn H. Encyclopedia of Parasitology.
    3rd Edition, Spinger, Heidelberg, Germany
2006
  • Julsing M. K., Quax W. J. and Kayser O. (2006)
    Engineering of Medicinal Plants - Prospects and Limitations of Medicinal Plant Biotechnology.
    in Kayser O., Quax W. J. Medicinal Plant Biotechnology - from Basic Research to Industrial Application.
    Wiley-VCH, Weinheim, Germany
Patent applications
2010
  • Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
    Biocatalytic oxidation process with alkL-gene product.
    WO 2011/131420 / PCT/EP2011/053834 / priority: 10 2010 015 807.0 20.04.2010 DE
Oral Presentations
2012
  • Julsing M. K.*, Schrewe M., Kuhn D., Cornelissen S., Bühler B. and Schmid A. (2012)
    Genome mining for biocatalytic oxyfunctionalizations: the oxygenase is not enough.
    GETGEOWEB - 1st Transnational Workshop, 29. - 30. October 2012, TU Bergakademie Freiberg, Germany
    * This person gave the talk.
Posters
2015
  • Willrodt C., Hoschek A., Julsing M. K. and Schmid A. (2015)
    Mixed-culture resting cell fermentation enables production of oxygenated monoterpenoids.
    Bioflavour 2015 - International Conference on Flavour and Fragrance Biotechnology, 9. - 11. September 2015, Frankfurt, Germany
  • Willrodt C., Hoschek A., Julsing M. K. and Schmid A. (2015)
    Synthetic biology for synthetic chemistry - Enabling Cascade Reactions by Modular Two-Strain Fermentations.
    Gordon Research Conference on Synthetic Biology, 28. June - 3. July 2015, Newry, ME, USA
2013
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst and process development for fermentative synthesis of monoterpene-derived natural product.
    1st European Conference on Natural Products, September 22-25 2013, Frankfurt am Main, Germany
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst and process development for the fermentative synthesis of monoterpene-derived natural product.
    Biotrans 2013, July 21st - July 25th 2013, Manchester, United Kingdom
  • Lange K., Schütz V, Czepnik M, Quentmeier A, Kayser O., Maaß M., Julsing M. K. and Schmid A. (2013)
    Biotransformation of cannabigerolic acid (CBGA) to tetrahydrocannabinolic acid (THCA) using cell free THCA synthase from Cannabis sativa.
    Biotrans 2013, July 21st - July 25th 2013, Manchester, United Kingdom
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2013)
    Reaction and catalyst engineering for whole-cell-based multistep ω-functionalization of renewables: Sustainable and efficient production of bifunctional building blocks for polymer syntheses.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Lange K., Julsing M. K., Bühler B. and Schmid A. (2013)
    Biocatalyst design for the production of cannabinoids.
    CLIB Retreat, February 13, 2013, Lünen, Germany
  • Willrodt C., David C., Julsing M. K., Bühler B. and Schmid A. (2013)
    Preparation of monoterpenes from cheap and renewable resources.
    Tag der Chemie, TU Dortmund, February 1st, 2013, Dortmund, Germany
2012
  • Lange K., Hoschek A., Bühler B., Julsing M. K. and Schmid A. (2012)
    Functional expression of the THCA synthase from Cannabis sativa in E. coli.
    Biocat 2012, August 31st - September 4th 2012, Hamburg, Germany
  • Willrodt C., Julsing M. K., Bühler B. and Schmid A. (2012)
    Monoterpenoid production in metabolically engineered Escherichia coli.
    Biocat 2012, August 31st - September 4th 2012, Hamburg, Germany
  • Ladkau N., Schrewe M., Julsing M. K., Bühler B. and Schmid A. (2012)
    Specific terminal oxy- and aminofunctionalization of dodecanoic acid methyl ester coupled in a single whole-cell catalyst.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL: a tool for the microbial biotransformation of hydrophobic substrates.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Rensmann L., Maaß M., Teubner N.C., Bühler B., Julsing M. K. and Schmid A. (2012)
    Enzyme titer and specific activity of recombinant human CYP450 1A1 and 2D6 in function of cell physiology in a whole-cell set-up.
    11th International symposium on cytochrome P450. Biodiversity and Biotechnology., 22. - 26. June 2012, Turin, Italy, http://p450-torino.it/
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2012)
    Resting cells of recombinant E. coli show high epoxidation yields on energy source and high sensitivity to product inhibition.
    Catalyzing Bio-Economy - Biocatalysts for Industrial Biotechnology, Annual meeting of the DECHEMA-VAAM-Section Biotransformations, 24. - 25. April 2012, Frankfurt am Main, Germany
  • Lange K., Julsing M. K., Bühler B. and Schmid A. (2012)
    Functional expression of the THCA synthase from Cannabis sativa in E. coli.
    CLIB Retreat, February 22-24, 2012, Bergisch Galdbach, Germany
2011
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Multi-step whole-cell biocatalysis for the specific functionalization of unactivated terminal C‑H bonds.
    BioTrends 2011, 8. – 9. December 2011, Dortmund, Germany
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2011)
    Improved biotransformation rates for hydrophobic substrates using ths outer membrane protein AlkL in E. coli.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Whole-cell biocatalysis for the controlled terminal functionalization of sp3 hybridized C‑H bonds.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Lange K., Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2011)
    Biocatalyst design for the synthesis of cannabinoids in a microbial host: metabolic engineering for the production of monoterpenoids.
    1st European Congress of Applied Biotechnology, September 25-29, 2011, Berlin, Germany
  • Lange K., Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2011)
    Production of cannabinoids in a microbial host: synthesis of THCA in E. coli.
    CLIB Retreat, April 7, 2011, Bonn, Germany
2010
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2010)
    Oxygenase catalyzed styrene epoxidation using non-growing E.coli cells in a two-liquid phase system.
    BioTrends 2010, 1. - 2. December 2010, Dortmund, Germany
2009
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2009)
    The potential of resting E. coli cells for oxygenase catalysis.
    Biotrans 2009, 5th – 9th July 2009, Bern, Switzerland
  • Julsing M. K., Kuhn D., Schmid A. and Bühler B. (2009)
    A comparison of resting and growing E. coli for oxygenase catalysis.
    DECHEMA Jahrestagung der Biotechnologen, 8. - 10. September 2009, Mannheim, Germany
2007
  • Cornelissen S., Julsing M. K., Bühler B. and Schmid A. (2007)
    Evaluation of different recombinant microorganisms expressing mammalian CYP1A1 for productive whole-cell biocatalysis.
    15th International Conference on Cytochromes P450, Biophysics and Functional Genomics, 17. - 21. July 2007, Bled, Slovenia


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