People

Dr. rer. nat. Manfred Schrewe

Dr. rer. nat. Manfred Schrewe
Dr. rer. nat. Manfred Schrewe
Department Solar Materials
Umweltforschungszentrum Leipzig
Gebäude 9.2 Kubus Raum 410
04318 Leipzig
Germany

Tel.: +49-341-235 4686
Fax.: +49-341-235-45 1286

E-Mail:



Resume

Manfred Schrewe, born 1978 in Paderborn (Germany), is a PostDoc in the research group Applied Biocatalysis.

After training as a biological technical assistant at the vocational college of the Hochsauerlandkreis in Olsberg he studied Molecular Biotechnology at the University of Bielefeld (2001-2006). In his diploma thesis at the Chair of Fermentation Engineering of Prof. E. Flaschel he worked on the improvement of recombinant protein production by means of process and genetic engineering. During his internship at the Department of Bioprocess Technology of Prof. S.O. Enfors at the Royal Institute of Technology (KTH), Stockholm, Sweden, he assisted in the development of a fingerprint method to characterize bacteria using SPR.

From March 2007 till June 2013, Manfred carried out his PhD thesis at the Laboratory of Chemical Biotechnology under supervision of Prof. A. Schmid and Dr. B. Bühler. In his research, he focused on whole-cell biocatalysis for the oxyfunctionalization of hydrocarbons. His thesis is entitled "Synthetic pathway and process engineering for terminal oxy- and aminofunctionalization via multistep biocatalysis in living microbial cells".

Next to the ongoing research on the production of functionalized hydrocarbons with whole cells, in his PostDoc project Manfred focuses on hydrogenase based catalysis for in vivo evolution of hydrogen and the utilization of H2 for cofactor regeneration, respectively.

Publications
Peer Reviewed Articles
2016
  • Kadisch M., Julsing M. K., Schrewe M., Jehmlich N., Scheer B., von Bergen M., Schmid A. and Bühler B. (2016)
    Cell viability rather than enzyme kinetics determines whole-cell ω-oxyfunctionalization performance.
    Biotechnology & Bioengineering, Accepted
  • Ladkau N., Aßmann M., Schrewe M., Julsing M. K., Schmid A. and Bühler B. (2016)
    Efficient production of the Nylon 12 monomer ω-aminododecanoic acid methyl ester from renewable dodecanoic acid methyl ester with engineered Escherichia coli.
    Metabolic Engineering, 36:1-9, DOI-Link
2014
  • Schrewe M., Julsing M. K., Lange K., Czarnotta E, Schmid A. and Bühler B. (2014)
    Reaction and catalyst engineering to exploit kinetically controlled whole-cell multistep biocatalysis for terminal FAME oxyfunctionalization.
    Biotechnology and Bioengineering, 111(9):1820-1830, DOI-Link
2013
  • Schrewe M., Ladkau N., Bühler B. and Schmid A. (2013)
    Direct terminal alkylamino-functionalization via multistep biocatalysis in one recombinant whole-cell catalyst.
    Advanced Synthesis and Catalysis, 355(9):1693-1697, DOI-Link
  • Schrewe M.*, Julsing M. K.*, Bühler B. and Schmid A. (2013)
    Whole-cell biocatalysis for selective and productive C-O functional group introduction and modification.
    Chemical Society Reviews, 42(15):6346 - 6377, DOI-Link
    * These authors contributed equally to this work.
2012
  • Julsing M. K.*, Schrewe M.*, Cornelissen S., Hermann I., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in E. coli.
    Applied and Environmental Microbiology, 78(16):5724-5733, DOI-Link
    * These authors contributed equally to this work.
2011
  • Schrewe M., Magnusson A., Willrodt C., Bühler B. and Schmid A. (2011)
    Kinetic analysis of terminal and unactivated C-H bond oxyfunctionalization in fatty acid methyl esters by monooxygenase-based whole-cell biocatalysis.
    Advanced Synthesis and Catalysis, 353:3485 – 3495, DOI-Link
Books & Bookchapters
2013
  • Schrewe M. (2013)
    Synthetic pathway and process engineering for terminal oxy- and aminofunctionalization via multistep biocatalysis in living microbial cells.
    Shaker Verlag GmbH, Herzogenrath, Germany, ISBN: 978-3-8440-2185-1, Book-Link
Patent applications
2010
  • Pötter M., Schmid A., Bühler B., Hennemann H.-G., Julsing M. K., Schaffer S., Haas T., Schrewe M., Cornelissen S., Roos M. and Häger H. (2010)
    Biocatalytic oxidation process with alkL-gene product.
    WO 2011/131420 / PCT/EP2011/053834 / priority: 10 2010 015 807.0 20.04.2010 DE
Oral Presentations
2013
  • Schrewe M., Ladkau N., Julsing M. K., Schmid A. and Bühler B. (2013)
    Multistep whole-cell biocatalysis: Bifunctional products from renewables.
    RSC symposium: High-value chemicals from biomass, 17. September 2013, London, UK
Posters
2014
  • Ladkau N., Schrewe M., Schmid A. and Bühler B. (2014)
    Three-step sequential whole-cell biocatalysis for the technical scale production of 12-aminododecanoic acid methyl ester from renewables.
    3rd Multistep Enzyme Catalyzed Processes Congress (MECP14), 7. - 10. April 2014, Madrid, Spain
2013
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2013)
    Reaction and catalyst engineering for whole-cell-based multistep ω-functionalization of renewables: Sustainable and efficient production of bifunctional building blocks for polymer syntheses.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
  • Ladkau N., Schrewe M., Aßmann M., Klassen G., Bühler B. and Schmid A. (2013)
    Strain engineering for the omega-aminofunctionalization of dodecanoic acid methyl ester via multistep biocatalysis.
    Biotrans 2013, 21. - 25. July 2013, Manchester, UK
2012
  • Ladkau N., Schrewe M., Julsing M. K., Bühler B. and Schmid A. (2012)
    Specific terminal oxy- and aminofunctionalization of dodecanoic acid methyl ester coupled in a single whole-cell catalyst.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2012)
    The outer membrane protein AlkL: a tool for the microbial biotransformation of hydrophobic substrates.
    Gordon Research Conference Biocatalysis, 8. - 13. July 2012, Smithfield, RI, USA
2011
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Multi-step whole-cell biocatalysis for the specific functionalization of unactivated terminal C‑H bonds.
    BioTrends 2011, 8. – 9. December 2011, Dortmund, Germany
  • Julsing M. K., Schrewe M., Cornelissen S., Schmid A. and Bühler B. (2011)
    Improved biotransformation rates for hydrophobic substrates using ths outer membrane protein AlkL in E. coli.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
  • Schrewe M., Ladkau N., Lange K., Czarnotta E, Julsing M. K., Bühler B. and Schmid A. (2011)
    Whole-cell biocatalysis for the controlled terminal functionalization of sp3 hybridized C‑H bonds.
    Biotrans 2011, 2. - 6. October 2011, Giardini Naxos, Sicily, Italy
2010
  • Ladkau N., Schrewe M., Bühler B. and Schmid A. (2010)
    Multistep-Biocatalysis for the production of 12-aminolauric acid methyl ester from lauric acid methyl ester with recombinant E. coli.
    EFB Bioprocess Engineering Course, 19. - 26. September 2010, Supetar, Croatia
2009
  • Schrewe M., Schmid A. and Bühler B. (2009)
    Application of a whole-cell biocatalyst for the omega-oxyfunctionalizationof fatty acid methyl ester.
    Biotrans 2009, 5th – 9th July 2009, Bern, Switzerland
2008
  • Schrewe M., Bühler B. and Schmid A. (2008)
    Development of a productive whole-cell biocatalyst for ω-hydroxylation of fatty acid methyl esters.
    Biocat 2008, 31st of August - 4th of September 2008, Hamburg, Germany